Effect of Fsh Β-sub Unit and Fshr Genes Polymorphisms on Superovulatory Response Traits

Follicle stimulating hormone (FSH) is a pituitary expressed glycoprotein hormone that regulates reproduction in mammals which composed of α and β-sub unit. The β-sub unit dictates its binding specificity with their receptor (FSHR). This study aimed to identify polymorphism of FSH β-sub unit and FSHR genes, and its effect to superovulatory response traits on superovulated cows. Study was done on 32 cows including Angus, Friesian Holstein (FH), Limousin, Simmental and Brahman in Cipelang Livestock Embryo Center. Cows used have been treated superovulation and mated by artificial insemination. Superovulation response (SR), ovulation rate (OR), fertilization percentage (FP) and viable transfer embryo percentage (VP) were analyzed to investigate the effect of FSH β-sub unit and FSHR polymorphism. Allele frequency of FSH β-sub unit|PstI and FSH|AluI were opposite within species. Mostly B allele and C allele for FSH β-sub unit and FSHR respectively have a high number in Bos taurus species while those were in contrast in Bos indicus species. The highest heterozygosity was found in FH cattle (0.250) for FSH β-sub unit and Brahman (0.333) for FSHR. Significant effect was FSH β-sub unit and FSHR Gene on Superovulatory Response Traits (E. Andreas et al.) 197 found between FSHR gene polymorphism with ovulation rate where CC genotype was higher (P<0.05) than CG and GG genotypes.